Abstract  Objective  Studying the brain biochemical metabolism of infant spasm through hydrogen proton magnetic resonance spectrum (¹H-MRS). Method  40 infant spasm in hospital and 10 age-matched normal infants were performed brain MRI plain scan and 1 H-MRS check by using 1.5 T superconducting MRI scanner. Choosing the same interesting region: Bilateral thalamus 、 basal ganglia region、 temporal lobe, getting spectral graph of brain tissue and relative concentration and ratio of  N-acetyl aspartic acid(NAA) 、choline(Cho)、creatine(Cr). Result  The ratio of NAA/Cho+Cr﹑NAA/Cho﹑NAA/Cr in Bilateral thalamus﹑basal ganglia region﹑temporal lobe of  infant spasm group were lower than normal group, P﹤0.01, the difference were statistically  significant.Compared bilateral symmetric areas of interest , the ratio of NAA/Cho+Cr﹑NAA/Cho﹑NAA/Cr had no significant difference in the two groups, P﹥0.05, the difference were not statistically  significant. Conclusion  ¹H-MRS can discover brain function change of Infant Spasm non-invasively; NAA in brain tissue of infant spasm were lower than normal group.